STREPTOKINASE TEST,MECANISUM AND PROTOCOLS

                              

                       STREPTOKINASE TEST

STREPTOKINASE  IS AN EXO ENZYME  DERIVED FROM  STREPTOCOCCI, IF THE SAME ENZYME DERIVED FROM  STAPHYLOCOCCUS AUREUS THEN IT IS KNOWN AS STAPHYLOKINAS.SINCE IT IS AN ENZYME IT ACCELARATES   THE REACTION.

STREPTOKINASE  IS AN ENZYME THAT CONVERT PLASMMINOGEN WHICH  IS AN ZYMOGEN i:e (ENYME WHICH IS IN INACTIVE FORM)  TO PLASMEN.PLASMINOGEN IS NORMAALLY PRESENT IN THE BLOOD  IN ZYMOZEN FROM WHEN THERE IS FIBRIN CLOT OR DEGRADATION FIBRINE THE PLASMINOGEN IS ATTRACTED TO FIBRIN CLOT AND CONVERTED INTO PLASMIN.IN  HUMAN WE HAVE TROMBIN WHICH CONVERT PLASMINOGEN TO PLASMIN,BUT IN SOME CONDITION IT DOES NOT WORK AND FIBRIN CLOT IS NOT DEGRADED AND CREATS OBSTRUCTION IN VEINS,ARTARY,BRAIN AND SO ON.                                                 

    IN NOMAAL EXTERNAL WOUND HEALING CONDITION PLATELET CLOT IS WEAK AND CAN PERMIT EXTERNAL PATHOGENIC MICROORGANISUM.IN ORDER TO STRENTHEN THE PLATELET CLOT THE FIBRIN COVERS ENTIER PLATELET CLOT AND FORMS FIBRIN CLOT.

            MECHANISUM  STREPTOKINASE  OF  ???????????  

AS WE KNOW PLASMINOGEN IS ZYMOGEN SO IT NEEDS THROMBIN TO CONVERT INTO PLASMEN AND PLASMIN DEGRADE THE BONDING BETWEEN FIBRIN AND CONVERT THEM INTO FIBRINOGEN.

IN THESE WAY STREPTOKINASE DOES THE FUNCTION OF THROMBIN AND CONVERTS FIBRIN TO FIBRINOGEN,AND BREAKS FIBRIN CLOT.

         STREPTOKINASE AS VIRULENCE  FACTOR ??????????????

AS WE KNOW THAT THESE ENZYME CAN BREAK FIBRIN INTO ITS SMALLER COMPONENTS SINCE IN WOUNDED STATE FIBRIN IS A BARRIER BETWEEN BLOOD AND ENVIRONMENT,AS IN CASE IF AN MICROORGANISUM ABLE TO PRODUCE STREPTOKINASE IS SETTLED ON UNHEALED WOUND CONTAINING FIBRIN CLOT,THEN THESE ORGANISUM  CAN EASILY PASS THROUGH  FIBRIN CLOT AND CAUSE THE RESPECTIVE DISEASE.BECAUSE OF THESE REASON IT IS AN VIRULENCE FACTOR.

                               -:PROTOCOLS FOR STREPTOKINASE TEST:- 

1)  NAME TWO SUSPENSION TUBE WITH NEGETIVE CONTROL AND TEST.

2)  SUSPEND EQUAL SIZE BLOOD CLOT IN BOTH SUSPENSION TUBE WITH 3 ml  STERILE SALINE.

3)  IN NEGETIVE CONTROL TUBE ADD 0.5 ml STERILE SALINE.

4)  IN TEST SUSPENSION TUBE ADD O.5 ml S.aureus AND S.pyogenes CULTURE SOLUTION.

5)  INCUBATE THE SUSPENSION TUBE AT 37 degree celsius.

   NOTE: DO NOT SHAKE SUSPENSION TUBE AFTER AND BEFORE INOCULATION.

                                         OBSERVATION AFTER 24 HOURS

• • SEE THE SIZE OF BLOOD CLOT AND OBSERVE THE DIMENSION OF BLOOD CLOT BEFORE AND AFTER.

    • 

      IF BLOOD CLOT SIZE INCREASE IT MEANS IT IS DEGRADED BUT IT IS SWOLLEN DUE TO WATER RETAINTION.                                                                         

     RESULT:-

1) S.aureus is stereptokinase positive.

2) S.pyogenes is stereptokinase positive.